1 Brightfield Microscopy

2 Darkfield Microscopy

3 Phase-Contrast Microscopy

4 Microscopic Measurements

Part 2 Survey of Microorganisms

5 Protozoa, Algae and Cyanobacteria

6 Ubiquity of Bacteria

7 The Fungi: Molds and Yeasts

Part 3 Manipulation of Microorganisms

8 Aseptic Technique

9 Pure Culture Techniques

Part 4 Staining and Observations of Microorganisms

10 Smear Preparation

11 Simple Staining

12 Negative Staining

13 Capsular Staining

14 Gram Staining

15 Spore Staining: Two Methods

16 Acid-Fast Staining: Kinyoun Method

17 Motility Determination

Part 5 Culture Methods

18 Culture Media Preparation

19 Enumeration of Bacteria: The Standard Plate Count

20 Slide Culture: Fungi

Part 6 Bacterial Viruses

21 Determination of a Bacteriophage Titer

22 Isolation of Phage from Flies

23 Phage Typing

Part 7 Environmental Influences and Control of Microbial Growth

24 Effects of Oxygen on Growth

25 Temperature: Effects on Growth

26 pH and Microbial Growth

27 Water Activity and Osmotic Pressure

28 Ultraviolet Light: Lethal Effects

29 The Effects of Lysozyme on Bacterial Cells

30 Evaluation of Alcohol: Its Effectiveness as an Antiseptic

31 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method

32 Evaluation of Antiseptics: The Filter Paper Disk Method

33 Effectiveness of Hand Scrubbing

Part 8 Identification of Unknown Bacteria

34 Morphological Study of An Unknown Bacterium

35 Cultural Characteristics

36 Physiological Characteristics: Oxidation and Fermentation Stet Reactions

37 Physiological Characteristics: Hydrolytic and Degradative Stet Reactions

38 Physiological Characteristics: Multiple Test Media

39 Use of Bergey’s Manual

Part 9 Miniaturized Multitest Systems

40 Enterobacteriaceae Identification: The API 20E System

41 Enterobacteriaceae Identification: The Enterotube II System

42 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System

43 Staphylococcus Identification: The API Staph System

Part 10 Applied Microbiology

44 Bacterial Food Counts

45 Bacteriological Examination of Water: Most Probable Number Determination

46 Bacteriological Examination of Water: The Membrane Filter Method

47 Reductase Test

48 Temperature: Lethal Effects

49 Microbial Spoilage of Canned Food

50 Microbiology of Alcohol Fermentation

Part 11 Medical Microbiology

51 The Staphylococci: Isolation and Identification

52 The Streptococci and Enterococci: Isolation and Identification

53 Gram-Negative Intestinal Pathogens

54 A Synthetic Epidemic

Part 12 Immunology and Serology

55 Slide Agglutination Test: Serological Typing

56 Slide Agglutination Test for S. aureus

57 Slide Agglutination Test for Streptococcus

58 Tube Agglutination Test: The Heterophile Antibody Test

59 Blood Grouping

Appendices