Hiroshi Ashihara, Department of Biological Sciences, Graduate School of Humanities and Sciences, Ochanomizu University, Tokyo, Japan.

Alan Crozier, Plant Products and Human Nutrition Group, School of Medicine, University of Glasgow, Glasgow, UK.

Atsushi Komamine, Kihara Instutute for Biological Research, Yokohama City University, Yokohama, Japan.

Preface xv

1 Biosynthesis and Metabolism of Starch and Sugars 1
Frederik Börnke and Sophia Sonnewald

1.1 Introduction 1

1.2 Carbon Partitioning in Mesophyll Cells 2

1.3 Sucrose Biosynthesis in Source Leaves 3

1.4 Starch Metabolism in Source Leaves 7

1.5 Sucrose to Starch Conversion in Storage Organs 12

1.6 Metabolic Engineering of Carbohydrate Metabolism 14

1.7 Engineering Soluble Sugars 16

1.8 Production of Novel Carbohydrates in Transgenic Plants 17

1.9 Network Analysis of Carbohydrate Metabolism 18

Acknowledgements 19

References 19

2 Lipid Biosynthesis 27
David Hildebrand

2.1 Introduction 27

2.2 Fatty Acid Synthesis 29

2.3 Fatty Acid Desaturases 32

2.4 Lipid Signals 38

2.5 Algae 38

2.6 Membrane Synthesis 39

2.7 TAG Biosynthesis 43

2.8 Genetic Engineering of Oilseed for Industrial Uses 46

2.9 Plant Oils as a Renewable Resource 48

Acknowledgements 51

References 52

3 Symbiotic Nitrogen Fixation 67
Hiroshi Kouchi

3.1 Nitrogen Fixing Organisms and the Nitrogenase System 67

3.2 Symbiotic Nodule Formation in Legume Plants 70

3.3 Mutual Interactions between Host Cells and Bacteroids in Legume Nodules 76

3.4 Molecular Genetic Approaches to the Host Regulation of Nitrogen Fixation 83

Acknowledgements 92

References 92

4 Sulfur Metabolism 103
Hideki Takahashi

4.1 Introduction 103

4.2 Sulfate Transport 104

4.3 Sulfate Reduction 111

4.4 Cysteine Biosynthesis 114

4.5 Methionine Biosynthesis 117

4.6 Regulators for Coordination of Sulfur Metabolism 118

References 121

5 Nucleotide Metabolism 135
Rita Zrenner and Hiroshi Ashihara

5.1 Introduction 135

5.2 Pyrimidine Metabolism 136

5.3 Purine Metabolism 142

5.4 Pyridine Metabolism 149

5.5 Biotechnological Approaches 152

References 156

6 Purine Alkaloid Metabolism 163
Hiroshi Ashihara, Shinjiro Ogita and Alan Crozier

6.1 Introduction 163

6.2 Classification of Purine Alkaloids 166

6.3 Occurrence of Purine Alkaloids 166

6.4 Biosynthesis of Caffeine 167

6.5 Catabolism of Caffeine 171

6.6 Physiological and Ecological Aspects of Purine Alkaloid Metabolism in Plants 173

6.7 Metabolic Engineering of Caffeine In Planta 176

References 184

7 Nicotine Biosynthesis 191
Tsubasa Shoji and Takashi Hashimoto

7.1 Introduction 191

7.2 Pathways and Enzymes 192

7.3 Compartmentation and Trafficking 200

7.4 Gene Regulation 203

7.5 Metabolic Engineering 207

7.6 Recent Developments 208

7.7 Summary 208

References 208

8 Terpenoid Biosynthesis 217
Dae-Kyun Ro

8.1 Introduction 217

8.2 Terpenoid Diversity 218

8.3 Mechanistic Aspects of Terpenoid Biogenesis 222

8.4 Terpene Synthase – Structure, Evolution and Engineering 223

8.5 Two Distinct Pathways for Isopentenyl Diphosphate (IPP) Biosynthesis 225

8.6 Subcellular and Cellular Compartmentalisations of Terpenoid Metabolism 228

8.7 Gene Clusters in Terpenoid Metabolism 230

8.8 Metabolic Engineering of Terpenoid Metabolism 231

8.9 Concluding Remarks 235

References 235

9 Benzylisoquinoline Alkaloid Biosynthesis 241
Isabel Desgagn´e-Penix and Peter J Facchini

9.1 Introduction 241

9.2 Biosynthesis 242

9.3 Localisation and Transport of Benzylisoquinoline Alkaloids and their Biosynthetic Enzymes 249

9.3.1 Cellular and Subcellular Localisation 249

9.3.2 Transport 251

9.4 Regulation 251

9.4.1 Gene Regulation 251

9.4.2 Signal Transduction 251

9.5 Application to Biotechnology 252

9.5.1 Mutagenesis 252

9.5.2 Genetic Transformation and Metabolic Engineering 252

9.5.3 Metabolic Engineering 254

9.6 Conclusions 254

References 254

10 Monoterpenoid Indole Alkaloid Biosynthesis 263
Vincenzo De Luca

10.1 Introduction 263

10.2 Monoterpenoid Indole Alkaloid (MIA) Biosynthesis 265

10.3 MIA Pathway Gene Discovery will be Enhanced by Large-Scale Sequencing and Comparative Analyses 271

10.4 Developmental and Environmental Regulation of MIA Biosynthesis 272

10.5 Metabolic Engineering using Enzymes with Altered Substrate Specificity 282

10.6 Conclusion 282

Acknowledgements 283

References 283

11 Flavonoid Biosynthesis 293
Indu B. Jaganath and Alan Crozier

11.1 Introduction 293

11.2 Advances in Molecular Approaches for Flavonoid Biosynthetic Pathway Elucidation 294

11.3 The Flavonoid Biosynthetic Pathway as it is Today 299

11.4 Conclusions 311

References 313

12 Pigment Biosynthesis I. Anthocyanins 321
Yoshihiro Ozeki, Yuki Matsuba, Yutaka Abe, Naoyuki Umemoto and Nobuhiro Sasaki

12.1 Introduction 321

12.2 The Anthocyanin Biosynthetic Pathway 322

12.3 Glycosylation of Anthocyanidins 325

12.4 Acylation of Anthocyanin Glycosides 327

12.5 Transport of Anthocyanins from Cytosol to Vacuoles 331

12.6 Concluding Remarks 335

References 336

13 Pigment Biosynthesis II: Betacyanins and Carotenoids 343
Masaaki Sakuta and Akemi Ohmiya

13.1 Betacyanins 343

13.2 Carotenoids 350

13.3 Metabolic Engineering of Carotenoids 357

References 361

14 Metabolomics in Plant Biotechnology 373
Yozo Okazaki, Akira Oikawa, Miyako Kusano, Fumio Matsuda and Kazuki Saito

14.1 Introduction 373

14.2 Analytical Technologies 373

14.3 Informatics Techniques 376

14.4 Biotechnological Application 378

Acknowledgements 381

References 381

Index 389 

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