Now in its second edition, this updated text examines the new advances made in this field since the publication of the first edition twelve years ago. New concepts presented include monolithic columns, bonded stationary phases, micro-HPLC, two-dimensional comprehensive liquid chromatography, gradient elution mode, retention models for ions, and capillary electromigration techniques. The book also addresses HPLC detectors, LC-MS interfaces, nonlinear chromatography, displacement chromatography of peptides and proteins, polymer HPLC, field-flow fractionation, affinity and ion chromatography. Significant areas of HPLC application are also explored, including chiral pharmaceutical, environmental, food, and forensic analysis. Explanatory figures, tables, and extensive references supplement the text.