Advanced Electroporation Techniques in Biology and Medicine
ISBN: 9781439819067 | 1439819068
Edition: 1stFormat: Hardcover
Publisher: CRC Press
Pub. Date: 6/2/2010
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A reflection of the intense study of the effects of electromagnetic fields on living tissues that has taken place during the last several decades, this book discusses the theoretical and experimental evidence and considerations the effects of strong electromagnetic fields and/or electric pulses and their importance in medicine and biology. The authors present the basic techniques applied in electroporation and the advanced methods for creation of nanopores, highlighting their basic science and clinical applications. Topics include nano electroporation, classic electroporaiton, experimental evidence for electroporation of living cells, and electroporation for cancer and wound healing.
| Editors | p. xiii |
| Contributors | p. xv |
| MATLAB“ Disclaimer | p. xix |
| Basics of Electroporation | |
| Physical Chemical Theory of Membrane Electroporation and Electrotransfer of Biogenic Agents | p. 3 |
| Bioelectric Effect of Intense Nanosecond Pulses | p. 19 |
| Induced Transmembrane Voltage-Theory, Modeling, and Experiments | p. 51 |
| Electroporation: A Review of Basic Problems i... MORE | p. 71 |
| Mechanisms of Electroporation in Lipid Systems | |
| Electrodeformation, Electroporation, and Electrofusion of Cell-Sized Lipid Vesicles | p. 97 |
| Fluorescent Methods in Evaluation of Nanopore Conductivity and Their Computational Validation | p. 123 |
| Electroporation of Lipid Membranes: Insights from Molecular Dynamics Simulations | p. 141 |
| Nanoscale Restructuring of Lipid Bilayers in Nanosecond Electric Fields | p. 161 |
| Mechanisms of Electroporation of Cells | |
| Nanopores: A Distinct Transmembrane Passageway in Electroporated Cells | p. 177 |
| Model of Cell Membrane Electroporation and Transmembrane Molecular Transport | p. 195 |
| Kinetics of Pore Formation and Disappearance in the Cell during Electroporation | p. 213 |
| The Pulse Intensity-Duration Dependency for Cell Membrane Electroporation | p. 239 |
| Mechanisms of Electroporation in Tissues | |
| Drug-Free, Solid Tumor Ablation by Electroporating Pulses: Mechanisms That Couple to Necrotic and Apoptotic Cell Death Pathways | p. 255 |
| Gene Electrotransfer: From Basic Processes to Preclinical Applications | p. 281 |
| Technical Considerations | |
| Modeling Electric Field Distribution In Vivo | p. 299 |
| Concepts of Electroporation Pulse Generation and Overview of Electric Pulse Generators for Cell and Tissue Electroporation | p. 323 |
| Generation of Ultrashort Pulses | p. 341 |
| Nanosecond Pulsed Electric Field Delivery to Biological Samples: Difficulties and Potential Solutions | p. 353 |
| Application of Electroporation | |
| Translation of Electroporation-Mediated DNA Delivery to the Clinic | p. 371 |
| Clinical Electrochemotherapy: The Italian Experience | p. 385 |
| Tumor Blood Flow-Modifying Effects of Electroporation and Electrochemotherapy-Experimental Evidence and Implications for the Therapy | p. 401 |
| Electrochemotherapy as Part of an Immunotherapy Strategy in the Treatment of Cancer | p. 425 |
| Combined Electrical Field and Ultrasound: A Nondrug-Based Method for Tumor Ablation | p. 431 |
| Combined Modality Therapy: Electrochemotherapy with Tumor Irradiation | p. 441 |
| Irreversible Electroporation in Medicine | p. 453 |
| Food and Biomaterials Processing Assisted by Electroporation | p. 463 |
| In Vivo Electroporation: An Important Injury Mechanism in Electrical Shock Trauma | p. 491 |
| Index | p. 503 |
| Table of Contents provided by Ingram. All Rights Reserved. |
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