9781118873656

Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made – particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols.

This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online.

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.

R. IAN FRESHNEY, PhD, is an honorary senior research fellow in the Centre for Oncology and Applied Pharmacology, part of the Cancer Research UK Beatson Laboratories at the University of Glasgow. He is the author or editor of numerous books and a world-renowned expert on cell culture techniques.

List of Figures, xix

List of Color Plates, xxiii

List of Tables, xxv

List of Protocols, xxvii

List of Minireviews, xxix

Preface and Acknlowledgments, xxxi

Abbreviations, xxxiii

About the Companion Website xxxviii

1. Introduction, 1

2. Biology of Cultured Cells, 19

3. Laboratory Design and Layout, 37

4. Equipment and Materials, 51

5. Aseptic Technique, 73

6. Safety, Bioethics, and Validation, 89

7. Culture Vessels and Substrates, 111

8. Defined Media and Supplements, 125

9. Serum-Free Media, 149

10. Preparation and Sterilization, 173

10. Supplementary Material*

11. Primary Culture, 207

11. Supplementary Material*

12. Subculture and Cell Lines, 235

13. Authentication and Validation, 259

14. Microbial Contamination, 289

15. Cryopreservation and Banking, 307

16. Cloning and Selection, 327

16. Supplementary Material*

17. Cell Sorting, 347

18. Cell Line Characterization, 359

19. Differentiation, 383

20. Three]Dimensional Culture 401

21. Scale]up and Automation, 427

22. Senescence, Immortalization, and Transformation, 455

23. Quantitation, 483

23 Supplementary Material*

24. Cytotoxicity, 513

24. Supplementary Material*

25. Culture of Specific Cell Types, 531

25. Supplementary Material*

26. Stem Cells, 571

26. Supplementary Material*

27. Training Programs, 591

28. Problem Solving 627

29. In Conclusion, 651

Supplementary Material*

Appendix III: Sources of Equipment and Material

Appendix IV: Suppliers and Other Resource

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Culture of Animal Cells

Culture of Animal Cells

1118873653

Summary

Author Biography

Table of Contents

Supplemental Materials