9780077510558

Laboratory Exercises in Microbiology, Ninth Edition was designed and written to be directly correlated to Prescott’s Microbiology, Ninth Edition, by Joanne M. Willey, Linda M. Sherwood, and Christopher J. Woolverton. The class-tested exercises are modular to allow instructors to easily incorporate them into their course. This balanced introduction to each area of microbiology now also has accompanying Connect content for additional homework and assessment opportunities.

PART ONE Microscopic Techniques
1. Bright-Field Light Microscope and Microscopic Measurement of Organisms
2. The Hanging Drop Slide and Bacterial Motility
3. Dark-Field Light Microscope
4. Phase-Contrast Light Microscope
5. Fluorescence Microscope
PART TWO Bacterial Morphology and Staining
6. Negative Staining
7. Smear Preparation and Simple Staining
8. Gram Stain
9. Acid-Fast Staining (Ziehl-Neelsen and Kinyoun) Procedures
10. Endospore Staining (Schaeffer-Fulton or Wirtz-Conklin)
11. Capsule Staining
12. Flagella Staining: West and Difco’s SpotTest Methods
PART THREE Basic Laboratory and Culture Techniques
13. Sterilization and Microbiological Culture Media Preparation
14. Culture Transfer Instruments, Techniques, and Isolation and Maintenance of Pure Cultures
15. Spread-Plate Technique
16. The Streak-Plate Technique: Selective and Differential Media
17. Pour-Plate Technique
18. Cultivation of Anaerobic Bacteria
19. Determination of Bacterial Numbers
PART FOUR Biochemical Activities of Bacteria
20. Carbohydrates I: Fermentation and β-Galactosidase Activity
21. Carbohydrates II: Triple Sugar Iron Agar Test
22. Carbohydrates III: Starch Hydrolysis
23. Lipids: Lipid Hydrolysis
24. Proteins, Amino Acids, and Enzymes I: Hydrogen Sulfide Production and Motility
25. Proteins, Amino Acids, and Enzymes II: The IMViC Tests
26. Proteins, Amino Acids, and Enzymes III: Casein Hydrolysis
27. Proteins, Amino Acids, and Enzymes IV: Gelatin Hydrolysis
28. Proteins, Amino Acids, and Enzymes V: Catalase Activity
29. Proteins, Amino Acids, and Enzymes VI: Coagulase and DNase Activity
30. Proteins, Amino Acids, and Enzymes VII: Oxidase Test and Bioluminescence
31. Proteins, Amino Acids, and Enzymes VIII: Urease Activity
32. Proteins, Amino Acids, and Enzymes IX: Lysine and Ornithine Decarboxylase Tests
33. Proteins, Amino Acids, and Enzymes X: Phenylalanine Deamination
34. Proteins, Amino Acids, and Enzymes XI: Nitrate Reduction
PART FIVE Rapid Multitest Systems
35. The API® 20E System
36. The Enterotube® II System
PART SIX Unknown Identification
37. Using the First Edition of Bergey’s Manual of Systematic Bacteriology to Identify Bacteria
38. General Unknown
PART SEVEN Environmental Factors Affecting Growth of Microorganisms
39. Temperature
40. pH
41. Osmotic Pressure
42. The Effects of Chemical Agents on Bacteria I: Disinfectants and Other Antimicrobial Products
43. The Effects of Chemical Agents on Bacteria II: Antimicrobial Agents (Kirby-Bauer Method) and the Etest®
44. Hand Washing, Environmental Sampling, and Microbiological Monitoring
45. Determination of a Bacterial Growth Curve: Classical and Two-Hour Methods
PART EIGHT Environmental and Food Microbiology
46. Standard Coliform Most Probable Number (MPN) Test and Colilert®-18 Coliform Test
47. Membrane Filter Technique for Coliforms and Fecal Streptococci; KONFIRMTM Test for Fecal Coliforms
48. Isolation of Escherichia coli Bacteriophages from Sewage and Determining Bacteriophage Titers
49. Enumeration of Soil Microorganisms
50. Bacterial Count of a Food Product
51. Examination of Milk for Bacteria
PART NINE Medical Microbiology
52. Hemagglutination Reactions: Blood Groups
53. Isolation of Normal Microbiota from the Human Body
54. Staphylococci
55. Pneumococci
56. Streptococci
57. Neisseriae
58. Aerobic and Anaerobic Endospore-Forming Bacteria
PART TEN Survey of Selected Eucaryotic Microorganisms
59. Fungi (Eumycota) I: Yeasts
60. Fungi (Eumycota) II: Zygomycota (Zygomycetes), Ascomycota (Ascomycetes), and Basidiomycota(Basidiomycetes)
PART ELEVEN Microbial Genetics and Genomics
61. Bacterial Mutation
62. Bacterial Transformation
63. Bacterial Conjugation: The Transfer of Antibiotic-Resistant Plasmids
64. Isolation of Genomic DNA from Baker’s Yeast (Saccharomyces cerevisiae)
65. Isolation and Purifi cation of Genomic DNA from Escherichia coli
PART TWELVE Scientific Inquiry
66. Independent Group Projects
67. Case Studies
Appendix A Dilutions with Sample Problems
Appendix B Transmission-Absorbance Table for Spectrophotometry
Appendix C Logarithms; pH and pH Indicators
Appendix D Scientifi c Notation
Appendix E Identifi cation Charts
Appendix F Reagents, Solutions, Stains, and Tests
Appendix G Culture Media Appendix H Sources and Maintenance of Microbiological Stock Cultures
Appendix I Laboratory Skills and Laboratory Thinking Skills
Appendix J Orientation to the Laboratory: Rules of Conduct and General Safety
Appendix K Summary of Universal Precautions and Laboratory Safety Procedures
Appendix L Biosafety Level (BSL)
Index

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Laboratory Exercises in Microbiology

Laboratory Exercises in Microbiology

0077510550

Summary

Table of Contents

1. Bright-Field Light Microscope and Microscopic Measurement of Organisms

2. The Hanging Drop Slide and Bacterial Motility

3. Dark-Field Light Microscope

4. Phase-Contrast Light Microscope

5. Fluorescence Microscope

PART TWO Bacterial Morphology and Staining

6. Negative Staining

7. Smear Preparation and Simple Staining

8. Gram Stain

9. Acid-Fast Staining (Ziehl-Neelsen and Kinyoun) Procedures

10. Endospore Staining (Schaeffer-Fulton or Wirtz-Conklin)

11. Capsule Staining

12. Flagella Staining: West and Difco’s SpotTest Methods

PART THREE Basic Laboratory and Culture Techniques

13. Sterilization and Microbiological Culture Media Preparation

14. Culture Transfer Instruments, Techniques, and Isolation and Maintenance of Pure Cultures

15. Spread-Plate Technique

16. The Streak-Plate Technique: Selective and Differential Media

17. Pour-Plate Technique

18. Cultivation of Anaerobic Bacteria

19. Determination of Bacterial Numbers

PART FOUR Biochemical Activities of Bacteria

20. Carbohydrates I: Fermentation and β-Galactosidase Activity

21. Carbohydrates II: Triple Sugar Iron Agar Test

22. Carbohydrates III: Starch Hydrolysis

23. Lipids: Lipid Hydrolysis

24. Proteins, Amino Acids, and Enzymes I: Hydrogen Sulfide Production and Motility

25. Proteins, Amino Acids, and Enzymes II: The IMViC Tests

26. Proteins, Amino Acids, and Enzymes III: Casein Hydrolysis

27. Proteins, Amino Acids, and Enzymes IV: Gelatin Hydrolysis

28. Proteins, Amino Acids, and Enzymes V: Catalase Activity

29. Proteins, Amino Acids, and Enzymes VI: Coagulase and DNase Activity

30. Proteins, Amino Acids, and Enzymes VII: Oxidase Test and Bioluminescence

31. Proteins, Amino Acids, and Enzymes VIII: Urease Activity

32. Proteins, Amino Acids, and Enzymes IX: Lysine and Ornithine Decarboxylase Tests

33. Proteins, Amino Acids, and Enzymes X: Phenylalanine Deamination

34. Proteins, Amino Acids, and Enzymes XI: Nitrate Reduction

PART FIVE Rapid Multitest Systems

35. The API® 20E System

36. The Enterotube® II System

PART SIX Unknown Identification

37. Using the First Edition of Bergey’s Manual of Systematic Bacteriology to Identify Bacteria

38. General Unknown

PART SEVEN Environmental Factors Affecting Growth of Microorganisms

39. Temperature

40. pH

41. Osmotic Pressure

42. The Effects of Chemical Agents on Bacteria I: Disinfectants and Other Antimicrobial Products

43. The Effects of Chemical Agents on Bacteria II: Antimicrobial Agents (Kirby-Bauer Method) and the Etest®

44. Hand Washing, Environmental Sampling, and Microbiological Monitoring

45. Determination of a Bacterial Growth Curve: Classical and Two-Hour Methods

PART EIGHT Environmental and Food Microbiology

46. Standard Coliform Most Probable Number (MPN) Test and Colilert®-18 Coliform Test

47. Membrane Filter Technique for Coliforms and Fecal Streptococci; KONFIRMTM Test for Fecal Coliforms

48. Isolation of Escherichia coli Bacteriophages from Sewage and Determining Bacteriophage Titers

49. Enumeration of Soil Microorganisms

50. Bacterial Count of a Food Product

51. Examination of Milk for Bacteria

PART NINE Medical Microbiology

52. Hemagglutination Reactions: Blood Groups

53. Isolation of Normal Microbiota from the Human Body

54. Staphylococci

55. Pneumococci

56. Streptococci

57. Neisseriae

58. Aerobic and Anaerobic Endospore-Forming Bacteria

PART TEN Survey of Selected Eucaryotic Microorganisms

59. Fungi (Eumycota) I: Yeasts

60. Fungi (Eumycota) II: Zygomycota (Zygomycetes), Ascomycota (Ascomycetes), and Basidiomycota(Basidiomycetes)

PART ELEVEN Microbial Genetics and Genomics

61. Bacterial Mutation

62. Bacterial Transformation

63. Bacterial Conjugation: The Transfer of Antibiotic-Resistant Plasmids