This color book and accompanying DVD introduce the basic principles of suturing and wound closure to laboratory animal technicians and staff performing surgery on research animals. They can also be used in a veterinary setting. The book and DVD provide descriptions, pictures, and videos depicting the importance of proper tissue and aseptic technique during wound closure, holding and handling of instruments, and needle placement on the needle driver. They also offer guidance on choice of needle and suture material, vessel ligation, the use of tissue staples, and more.