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Principles of Gene Manipulation and Genomics: Principles and Applications of Recombinant DNA an...

ISBN: 9781405156660 | 140515666X

Edition: 8th
Format: Paperback
Publisher: John Wiley & Sons Inc
Pub. Date: 12/11/2012

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Summary Table of Contents Author Biography

Written by the best-selling author team of Sandy Primrose and Richard Twyman, Gene and Genome Technology reflects the rapid progress in recombinant DNA technology over the last few years, its biological background and applications. The authors carefully weave their expertise on the increasing integration between gene manipulation and genomics with coverage of techniques, genome analysis, transcriptomics, proteomics, and metabolomics in a highly accessible and student-focused edition. The text includes material and research presented in the rece... MOREntly published Principles of Gene Manipulation and Genomics, Seventh Edition, with additional explanation, four-color illustrations, and helpful pedagogical features designed to help undergraduate students encountering advanced topics for the first time. Text boxes in every chapter offer insights into the history, methodology, ethical concerns, and applications of genomic technologies and Background Biology panels assist students needing background or refresher information on the essential biology. Thought-provoking end of chapter questions, summaries and a dedicated website (www.blackwellpublishing.com/genetech) provide further instruction and resources for both the student and instructor. All art from the book is available for download online and on CD-ROM.

In the decade following the publication of the human genome sequence, we have witnessed a revolution in recombinant DNA technology and its applications. The use of gene manipulation techniques on an ever-increasing scale has underpinned numerous advances in biotechnology and medicine, and has vastly increased our understanding of the world around us. Now in its eighth edition, Principles of Gene Manipulation and Genomics embraces this revolution, providing integrated coverage of the techniques used for gene manipulation, genomics, and its related disciplines. As in previous editions, the text covers the basic gene manipulation techniques and then describes recent developments in recombinant DNA technology, genome analysis, functional genomics, and proteomics, bringing these themes together in a final section that discusses how the techniques are applied in the real world.The eighth edition includes a number of new features such as full-color illustrations throughout, color-coded text boxes in each chapter offering insights into the history, methodology, ethical concerns, and applications of genomic technologies, chapter summaries and thought-provoking end-of-chapter questions. The book is accompanied by a dedicated website providing further instruction and resources for both the student and instructor as well as regular updates on important topics. All art from the book is available to instructors for download from the website.Principles of Gene Manipulation and Genomics is an accessible and up-to-date guide to this exciting field, and is the essential reference for upper-level undergraduate and graduate students of genetics, genomics, molecular biology, and recombinant DNA technology.

List of plates.Preface.Abbreviations.1 Gene manipulation in the post-genomics era.Part I Fundamental Techniques of Gene Manipulation.2 Basic techniques.3 Cutting and joining DNA molecules.4 Basic biology of plasmid and phage vectors.5 Cosmids, phasmids, and other advanced vectors.6 Gene-cloning strategies.7 Sequencing genes and short stretched of DNA.8 Changing genes: site-directed mutagenesis and protein engineering.9 Bioinformatics.Part II Manipulating DNA in Microbes, Animals, and Plants.10 Cloning in bacteria other than Escherichia coli.11 ... MORE

Sandy Primrose was an academic for 10 years and a senior manager in the biotechnology industry for 20 years. He now manages a consultancy practice focused on technology management.

Richard Twyman was an academic for 9 years and now manages a company that provides expert scientific writing and project management services. He is also a Visiting Fellow at the Department of Biology, University of York.

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