9780470610749

This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigators purpose, whether its basic science investigation, creation of disease models and screens, or cells for therapeutic applications.

Uma Lakshmipathy is a principal investigator at Life Technologies. She has a PhD in life sciences, with academic and industry experience in molecular biology and stem cells. Dr. Lakshmipathy holds four patents and has authored more than forty publications. Bhaskar Thyagarajan is a program manager at Life Technologies. He has a PhD in pharmacology, with expertise in the areas of molecular biology, DNA recombination, gene and cell therapy, and protein purification. He holds one patent and has authored more than twenty publications.

Foreword	p. vii
Preface	p. ix
Contributors	p. xi
Cloning and Gene Delivery	p. 1
DNA Assembly Technologies Based on Homologous Recombination	p. 3
Multigene Assembly for Construction of Synthetic Operons: Creation and Delivery of an Optimized AU-LN-One Expression Construct for Generating Mouse iPS Cells	p. 19
Strategies for the Delivery of Naked DNA	p. 37
Nonintegrating Technologies	p. 49
Episomal Vectors	p. 51
Nonintegrating DNA Virus	p. 85
Nonintegrating RNA Viruses	p. 103
Protein Delivery	p. 119
Integrating Technologies	p. 135
Sleeping Beauty Transposon-Mediated Stable Gene Delivery	p. 137
Integrating Viral Vectors for Gene Modifications	p. 163
Bacteriophage Integrases for Site-Specific Integration	p. 199
Improving Gene Targeting Efficiency in Human Pluripotent Stem Cells	p. 211
Applications	p. 227
Modified Stem Cells as Disease Models and in Toxicology Screening	p. 229
Screening and Drug Discovery	p. 251
Index	p. 273
Table of Contents provided by Ingram. All Rights Reserved.

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Primary and Stem Cells Gene Transfer Technologies and Applications

Primary and Stem Cells Gene Transfer Technologies and Applications

0470610743

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