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Microbiology Experiments : A Health Science Perspective

ISBN: 9780072476248 | 0072476249
Edition: 4th
Format: Paperback
Publisher: McGraw-Hill Science/Engineering/Math
Pub. Date: 2/14/2003

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SummaryTable of Contents
All experiments are correlated to Nester's Microbiology: A Human Perspective, 4/e, but can be used with any non-majors/allied health microbiology textbook.
Prefacevii
Laboratory Safetyviii
Laboratory Safety Agreementix
PART ONE Basic Microbiology
1(146)
... MORE
Introduction to Microbiology
1(2)
Ubiquity of Microorganisms
3(6)
Bright-field Light Microscopy, Including History and Working Principles
9(12)
Microscopic (Bright-field and Dark-field) Determination of Cell Motility, Form, and Viability Using Wet Mount and Hanging Drop Preparations
21(10)
Introduction to Staining of Microorganisms
29(2)
Simple Stains: Positive and Negative Stains
31(6)
Multiple and Differential Stains
37(14)
Introduction to Microbial Growth
49(2)
Pure Culture and Aseptic Technique
51(6)
Defined, Undefined, Selective, and Differential Media
57(4)
Quantification of Microorganisms
61(10)
Introduction to the Environment and Microbial Growth
69(2)
Aerobic and Anaerobic Growth
71(4)
The Effect of Incubation Temperature on Generation Time
75(10)
Introduction to Control of Microbial Growth
83(2)
Moist and Dry Heat Sterilization: Thermal Death Point and Thermal Death Time
85(10)
Control of Microbial Growth with Ultraviolet Light
95(6)
Osmotic Pressure, and Its Effect on the Rate and Amount of Microbial Growth
101(8)
Antiseptics and Antibiotics
109(12)
Introduction to Microbial Genetics
119(2)
Selection of Bacterial Mutants Resistant to Antibiotics
121(6)
Transformation: A Form of Genetic Recombination
127(6)
Bacterial Conjugation
133(8)
Gene Regulation: Induction and Catabolite Repression
141(6)
PART TWO The Other Microbial World
147(44)
Introduction to the Other Microbial World
147(4)
Microscopic Identification of Fungi
151(18)
Parasitology: Protozoa and Helminths
169(14)
Prokaryotic Viruses: Bacteriophage Isolation and Titering
183(8)
PART THREE Microbes and Humans
191(28)
Introduction to Medical Microbiology
191(2)
Normal Skin Flora
193(6)
Respiratory Microorganisms
199(6)
Identification of Enteric Gram-Negative Rods
205(6)
Clinical Unknown Identification
211(8)
PART FOUR Immunology
219(52)
Introduction to Some Immunological Principles and Techniques
219(2)
Differential White Blood Cell Stains
221(6)
Lysozyme, an Enzymatic Form of Natural Resistance
227(6)
Traditional Agglutination Reactions Employing Microbial and Nonmicrobial Antigens
233(12)
Lancefield Grouping of Pathogenic Streptococci with a Latex Slide Agglutination Test
245(8)
Use of an Enzyme-Linked Immunosorbent Assay (ELISA) Test for Coccidioides immitis Identification
253(8)
An Ouchterlony Double Immunodiffusion Test for Coccidioides immitis Identification
261(10)
PART FIVE Public Health
271(28)
Introduction to the Preention and Control of Communicable Diseases
271(2)
Epidemiology: A Staphylococcus Carrier Study
273(12)
Bacteriological Examination of Water: Multiple-Tube Fermentation and Membrane Filter Techniques
285(14)
PART SIX Applications of Biotechnology
299(14)
Introduction to Biotechnology
299(2)
Identifying DNA with Restriction Enzymes
301(6)
Identification of Bacteria Using the Ribosomal Data Project
307(6)
PART SEVEN Projects
313(24)
Introduction to the Individual Projects
313(2)
Hydrocarbon-Degrading Bacteria, Cleaning Up After Oil Spills
315(2)
Luminescent Bacteria: Bacteria That Produce Light
317(2)
Methylotrophs, Organisms That Grow on One-Carbon Compounds
319(2)
Deinococcus, Bacteria with Out-Of-this-World Capabilities
321(16)
APPENDICES
1. Living Microorganisms (Bacteria, Fungi, Protozoa, and Helminths) Chosen for Study in this Manual
323(2)
2. Dilution Practice Problems
325(2)
3. Metric System, Use of with Conversions to the English System of Measurement
327(2)
4. pH Adjustment of Liquid and Agar Growth Media
329(2)
5. Use of the Ocular Micrometer for Measurement of Relative and Absolute Cell Size
331(2)
6. Use of the Hemocytometer for Determining Total Cell Number in a Liquid Suspension
333(2)
7. Preparation of Covered Slide Cultures for Study of Intact Structure of a Mold Thallus
335(2)
Index337

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