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Microbiology Experiments : A Health Science Perspective

by: Kleyn, John; Bicknell, Mary

Microbiology Experiments : A Health Science Perspective

by: Kleyn, John; Bicknell, Mary

ISBN 13:
9780072476248
ISBN 10:
0072476249
Edition: 4th
Format: Paperback
Copyright: 02/14/2003
Publisher: McGraw-Hill Science/Engineering/Math
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Summary

All experiments are correlated to Nester's Microbiology: A Human Perspective, 4/e, but can be used with any non-majors/allied health microbiology textbook.

Preface

vii

Laboratory Safety

viii

Laboratory Safety Agreement

PART ONE Basic Microbiology

(146)

Introduction to Microbiology

(2)

Ubiquity of Microorganisms

(6)

Bright-field Light Microscopy, Including History and Working Principles

(12)

Microscopic (Bright-field and Dark-field) Determination of Cell Motility, Form, and Viability Using Wet Mount and Hanging Drop Preparations

(10)

Introduction to Staining of Microorganisms

(2)

Simple Stains: Positive and Negative Stains

(6)

Multiple and Differential Stains

(14)

Introduction to Microbial Growth

(2)

Pure Culture and Aseptic Technique

(6)

Defined, Undefined, Selective, and Differential Media

(4)

Quantification of Microorganisms

(10)

Introduction to the Environment and Microbial Growth

(2)

Aerobic and Anaerobic Growth

(4)

The Effect of Incubation Temperature on Generation Time

(10)

Introduction to Control of Microbial Growth

(2)

Moist and Dry Heat Sterilization: Thermal Death Point and Thermal Death Time

(10)

Control of Microbial Growth with Ultraviolet Light

(6)

Osmotic Pressure, and Its Effect on the Rate and Amount of Microbial Growth

101

(8)

Antiseptics and Antibiotics

109

(12)

Introduction to Microbial Genetics

119

(2)

Selection of Bacterial Mutants Resistant to Antibiotics

121

(6)

Transformation: A Form of Genetic Recombination

127

(6)

Bacterial Conjugation

133

(8)

Gene Regulation: Induction and Catabolite Repression

141

(6)

PART TWO The Other Microbial World

147

(44)

Introduction to the Other Microbial World

147

(4)

Microscopic Identification of Fungi

151

(18)

Parasitology: Protozoa and Helminths

169

(14)

Prokaryotic Viruses: Bacteriophage Isolation and Titering

183

(8)

PART THREE Microbes and Humans

191

(28)

Introduction to Medical Microbiology

191

(2)

Normal Skin Flora

193

(6)

Respiratory Microorganisms

199

(6)

Identification of Enteric Gram-Negative Rods

205

(6)

Clinical Unknown Identification

211

(8)

PART FOUR Immunology

219

(52)

Introduction to Some Immunological Principles and Techniques

219

(2)

Differential White Blood Cell Stains

221

(6)

Lysozyme, an Enzymatic Form of Natural Resistance

227

(6)

Traditional Agglutination Reactions Employing Microbial and Nonmicrobial Antigens

233

(12)

Lancefield Grouping of Pathogenic Streptococci with a Latex Slide Agglutination Test

245

(8)

Use of an Enzyme-Linked Immunosorbent Assay (ELISA) Test for Coccidioides immitis Identification

253

(8)

An Ouchterlony Double Immunodiffusion Test for Coccidioides immitis Identification

261

(10)

PART FIVE Public Health

271

(28)

Introduction to the Preention and Control of Communicable Diseases

271

(2)

Epidemiology: A Staphylococcus Carrier Study

273

(12)

Bacteriological Examination of Water: Multiple-Tube Fermentation and Membrane Filter Techniques

285

(14)

PART SIX Applications of Biotechnology

299

(14)

Introduction to Biotechnology

299

(2)

Identifying DNA with Restriction Enzymes

301

(6)

Identification of Bacteria Using the Ribosomal Data Project

307

(6)

PART SEVEN Projects

313

(24)

Introduction to the Individual Projects

313

(2)

Hydrocarbon-Degrading Bacteria, Cleaning Up After Oil Spills

315

(2)

Luminescent Bacteria: Bacteria That Produce Light

317

(2)

Methylotrophs, Organisms That Grow on One-Carbon Compounds

319

(2)

Deinococcus, Bacteria with Out-Of-this-World Capabilities

321

(16)

APPENDICES

1. Living Microorganisms (Bacteria, Fungi, Protozoa, and Helminths) Chosen for Study in this Manual

323

(2)

2. Dilution Practice Problems

325

(2)

3. Metric System, Use of with Conversions to the English System of Measurement

327

(2)

4. pH Adjustment of Liquid and Agar Growth Media

329

(2)

5. Use of the Ocular Micrometer for Measurement of Relative and Absolute Cell Size

331

(2)

6. Use of the Hemocytometer for Determining Total Cell Number in a Liquid Suspension

333

(2)

7. Preparation of Covered Slide Cultures for Study of Intact Structure of a Mold Thallus

335

(2)

Index

337

Amazon no longer offers textbook rentals. We do!

Microbiology Experiments : A Health Science Perspective

Microbiology Experiments : A Health Science Perspective

9780072476248

0072476249

Summary

Table of Contents

Supplemental Materials

Write a Review

Amazon no longer offers textbook rentals. We do!

Microbiology Experiments : A Health Science Perspective

Microbiology Experiments : A Health Science Perspective

9780072476248

0072476249

Summary

Table of Contents

Supplemental Materials

Write a Review

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