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An increasingly popular analytical method, hydrophilic interaction chromatography (HILIC) has the ability to retain and separate polar compounds that are often difficult to analyze by reversed-phase high-performance liquid chromatography (HPLC) or other analytical methods. Offering a comprehensive review, this book offers a fundamental understanding of how HILIC works. With contributions from leading analytical scientists who have extensive experience in HILIC as well as HPLC and step-by-step guidance for developing efficient, sensitive, and robust HILIC methods, this book serves as a practical guide for researchers.