9780131742529

THEdefinitive genetics lab manual for over 50 years, this user-friendly volume stresses classical genetics, but includes some of the recent advances related to molecular and human genetics as well.Drosophilaand Maize Experiments in Genetics: Monohybrid Crosses; Dihybrid Crosses. Cell Reproduction: Mitosis. Meiosis in Animals: Oogenesis and Spermatogenesis. Meiosis in Angiosperms: Microsporogenesis and Megasporogenesis. Polytene Chromosomes fromDrosophilaSalivary Glands. Sex Chromosomes and Gene Transmission. The Sex Check: A Study of Sex Chromatin in Human Cells. Human Chromosomes. Linkage and Crossing Over. Genetics of Ascospore Color inSordaria: An Investigation of Linkage and Crossing Over Using Tetrad Analysis. Open-Ended Experiments UsingDrosophila: Locating a Mutant Gene in Its Chromosome. Isolation of DNA. Restriction Endonuclease Digestion and Gel Electrophoresis of DNA. Amplification of DNA Polymorphisms by Polymerase Chain Reaction (PCR) and DNA Fingerprinting. Transformation ofEscherichia coli.Gene Action: Synthesis of ... b-Galactosidase inEscherichia coli.Chromatographic Characterization ofDrosophilamelanogaster Mutants. Bacterial Mutagenesis. Gene Recombination in Phage. Polygenic Inheritance: Fingerprint Ridge Count. Population Genetics: The Hardy-Weinberg Principle; The Effects of Selection and Genetic Drift. Applied Human Genetics.For anyone interested in hands-on genetics work.

Preface

v

Drosophila and Maize Experiments in Genetics: Monohybrid and Dihybrid Crosses

1

(18)

Principles of Probability

19

(8)

The Chi-Square Test

27

(14)

Cell Reproduction: Mitosis

41

(8)

Meiosis in Animals: Oogenesis and Spermatogenesis

49

(8)

Meiosis in Angiosperms: Microsporogenesis

57

(14)

Polytene Chromosomes from Drosophila Salivary Glands

71

(4)

Sex Chromosomes and Gene Transmission

75

(10)

The Sex Check: A Study of Sex Chromatin in Human Cells

85

(6)

Human Chromosomes

91

(32)

Linkage and Crossing Over

123

(20)

Genetics of Ascospore Color in Sordaria: An Investigation of Linkage and Crossing Over Using Tetrad Analysis

143

(8)

Open-Ended Experiments Using Drosophila: Locating a Mutant Gene in Its Chromosome

151

(6)

The Genetic Material: Isolation of DNA

157

(6)

Restriction Endonuclease Digestion and Gel Electrophoresis of DNA

163

(16)

The Amplification of DNA Polymorphisms by Polymerase Chain Reaction (PCR) and DNA Fingerprinting

179

(16)

Transformation of Escherichia coli

195

(10)

Gene Action: Synthesis of β-Galactosidase in Escherichia coli

205

(6)

Chromatographic Characterization of Drosophila melanogaster Mutants

211

(8)

Bacterial Mutagenesis

219

(6)

Gene Recombination in Phage

225

(6)

Polygenic Inheritance: Fingerprint Ridge Count

231

(10)

Population Genetics: The Hardy-Weinberg Principle

241

(12)

Population Genetics: The Effects of Selection and Genetic Drift

253

(8)

Applied Human Genetics

261

(20)

NCBI and Genomic Data Mining

281

(18)

Supplemental Laboratory Topics

299

(1)

Supplement 1 Plant Speciation by Interspecific Hybridization and Allotetraploidy

299

(2)

Supplement 2 Chromosome Translocations in Rhoeo spathacea

301

(1)

Supplement 3 Human Pedigree Analysis

302

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Genetics Laboratory Investigations

Genetics Laboratory Investigations

0131742523

Summary

Table of Contents

Supplemental Materials