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Part One: Basic Microbiology

Introduction to Microbiology

Exercise: 1 Ubiquity of Microorganisms

Exercise: 2 Bright-Field Light Microscopy, Including History and Working Principles

Exercise: 3 Microscopic (Bright-Field and Dark-Field) Determination of Cell Motility, Form, and Viability Using Wet Mount and Hanging Drop Preparations

Introduction to Staining of Microorganisms

Exercise: 4 Simple Stains: Positive and Negative Stains

Exercise: 5 Differential and Special StainsIntroduc... MOREtion to Microbial Growth

Exercise: 6 Pure Culture and Aseptic Technique

Exercise: 7 Chemically Defined, Complex, Selective, and Differential Media

Exercise: 8 Quantification of Microorganisms

Introduction to the Environment and Microbial Growth

Exercise: 9 Aerobic and Anaerobic Growth

Exercise: 10 The Effect of Incubation Temperature on Generation Time

Introduction to Control of Microbial Growth

Exercise: 11 Moist and Dry Heat Sterilization: Thermal Death Point and Thermal Death Time

Exercise: 12 Control of Microbial Growth with Ultraviolet Light

Exercise: 13 Osmotic Pressure, and Its Effect on Rate and Amount of Microbial Growth

Exercise: 14 Antiseptics and Antibiotics

Introduction to Microbial Genetics

Exercise: 15 Selection of Bacterial Mutants Resistant to Antibiotics

Exercise: 16 Transformation: A Form of Genetic Recombination

Exercise: 17 Bacterial Conjugation

Exercise: 18 Gene Regulation: Induction and Catabolite Repression

Part Two: The Other Microbial World

Introduction to the Other Microbial World

Exercise: 19 Microscopic Identification of Fungi

Exercise: 20 Parasitology: Protozoa and Helminths

Exercise: 21 Titering Prokaryotic Viruses

Part Three: Microbes and Humans

Introduction to Medical Microbiology

Exercise: 22 Normal Skin Flora

Exercise: 23 Respiratory Microorganisms

Exercise: 24 Identification of Enteric Gram-Negative Rods

Exercise: 25 Clinical Unknown Identification

Part Four: Immunology

Introduction to Some Immunological Principles and Techniques

Exercise: 26 Differential White Blood Cell Stains

Exercise: 27 Lysozyme, an Enzymatic Form of Natural Resistance

Exercise: 28 Lancefield Grouping of Pathogenic Streptococci with a Latex Slide Agglutination Test

Exercise: 29 Use of an Enzyme-Linked Immunosorbent Assay (ELISA) Test for Coccidioides Immitis Identification

Exercise: 30 An Ouchterlony Double Immunodiffusion Test for Coccidioides Immitis Identification

Part Five: Public Health

Introduction to the Prevention and Control of Communicable Diseases

Exercise: 31 Epidemiology: A Staphylococcus Carrier Study

Exercise: 32 Bacteriological Examination of Water: Multiple-Tube Fermentation and Membrane Filter Techniques

Part Six: Applications of Biotechnology

Introduction to Biotechnology

Exercise: 33 Identifying DNA with Restriction Enzymes

Exercise: 34 Identification of Bacteria Using the Ribosomal Data Project

Part Seven: Projects

Introduction to the Individual Projects

Exercise: 35 Hydrocarbon-Degrading Bacteria, Cleaning Up After Oil Spills

Exercise: 36 Luminescent Bacteria: Bacteria That Produce Light

Exercise: 37 Methylotrophs, Organisms That Grow on One-Carbon Compounds

Exercise: 38 Deinococcus, Bacteria with Out-Of-This-World Capabilities

Appendices

1. Living Microorganisms (Bacteria, Fungi, Protozoa, and Helminths) Chosen for Study in this Manual

2. Dilution Practice Problems

3. Metric System, Use of with Conversions to the English System of Measurement

4. Alternative Procedures

5. Use of the Ocular Micrometer for Measurement of Relative and Absolute Cell Size

6. Use of the Hemocytometer for Determining Total Cell Number in a Liquid Suspension

7. Preparation of Covered Slide Cultures for Study of Intact Structure of a Mold Thallus

8. Additional Reading