1 Brightfield Microscopy

2 Darkfield Microscopy

3 Phase-Contrast Microscopy

4 Fluorescence Microscopy

5 Microscopic Measurements

Part 2 Survey of Microorganisms

6 Protozoa, Algae and Cyanobacteria

7 Ubiquity of Bacteria

8 The Fungi: Molds and Yeasts

Part 3 Manipulation of Microorganisms

9 Aseptic Technique

10 Pure Culture Techniques

Part 4 Staining and Observations of Microorganisms

11 Smear Preparation

12 Simple Staining

13 Negative Staining

14 Capsular Staining

15 Gram Staining

16 Spore Staining: Two Methods

17 Acid-Fast Staining: Kinyoun Method

18 Motility Determination

Part 5 Culture Methods

19 Culture Media Preparation

20 Preparation of Stock Cultures

21 Enumeration of Bacteria: The Standard Plate Count

22 Slide Culture: Fungi

Part 6 Bacterial Viruses

23 Determination of a Bacteriophage Titer

24 Burst Size Determination: A One-Step Growth Curve

25 Isolation of Phage from Flies

26 Phage Typing

Part 7 Environmental Influences and Control of Microbial Growth

27 Effects of Oxygen on Growth

28 Temperature: Effects on Growth

29 pH and Microbial Growth

30 Water Activity and Osmotic Pressure

31 Ultraviolet Light: Lethal Effects

32 The Effects of Lysozyme on Bacterial Cells

33 Evaluation of Alcohol: Its Effectiveness as an Antiseptic

34 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method

35 Evaluation of Antiseptics: The Filter Paper Disk Method

36 Effectiveness of Hand Scrubbing

Part 8 Identification of Unknown Bacteria

37 Morphological Study of An Unknown Bacterium

38 Cultural Characteristics

39 Physiological Characteristics: Oxidation and Fermentation Stet Reactions

40 Physiological Characteristics: Hydrolytic and Degradative Stet Reactions

41 Physiological Characteristics: Multiple Test Media

42 Use of Bergey’s Manual

Part 9 Miniaturized Multitest Systems

43 Enterobacteriaceae Identification: The API 20E System

44 Enterobacteriaceae Identification: The Enterotube II System

45 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System

46 Staphylococcus Identification: The API Staph System

Part 10 Diversity and Environmental Microbiology

47 Isolation of an Antibiotic Producer: The Actinomyces

48 Nitrogen Cycle: Ammonification

49 Symbiotic Nitrogen Fixation: Rhizobium

50 Free-Living Nitrogen Fixation: Azotobacter

51 Denitrification: Paracoccus denitrificans

52 The Winogradsky Column

53 Purple Nonsulfur Photosynthetic Bacteria

54 Sulfate Reducing Bacteria: Desulfovibrio

55 Bacterial Commensalism

56 Bacterial Synergism

57 Microbial Antagonism

Part 11 Applied Microbiology

58 Bacterial Food Counts

59 Bacteriological Examination of Water: Most Probable Number Determination

60 Bacteriological Examination of Water: The Membrane Filter Method

61 Reductase Test

62 Temperature: Lethal Effects

63 Microbial Spoilage of Canned Food

64 Microbiology of Alcohol Fermentation

Part 12 Bacterial Genetics and Biotechnology

65 Mutant Isolation by Replica Plating

66 Bacterial Transformation

67 Polymerase Chain Reaction for Amplifying DNA

68 Plasmid Isolation

Part 13 Medical Microbiology

69 The Staphylococci: Isolation and Identification

70 The Streptococci and Enterococci: Isolation and Identification

71 Gram-Negative Intestinal Pathogens

72 A Synthetic Epidemic

Part 14 Immunology and Serology

73 Slide Agglutination Test: Serological Typing

74 Slide Agglutination Test for S. aureus

75 Slide Agglutination Test for Streptococcus

76 Tube Agglutination Test: The Heterophile Antibody Test

77 Blood Grouping

Appendices